High grade B-Cell Lymphoma


High-grade B-cell lymphomas are tumors of B-cells with aggressive appearing histology that encompass a variety of disease entities using current WHO criteria, including diffuse large B-cell lymphoma, Burkitt lymphoma, high-grade B-cell lymphoma, NOS, etc.  Importantly, it is necessary to identify and/or exclude the presence MYC and BCL2 and/or BCL6 rearrangements in these tumors as the results dictate therapy in most patients.  


  • Completed CGL Lymphoid Testing requisition form
  • FFPE Tumour specimen (see Specimen GuidelinesCytogenetics FISH FFPE test type)
    • An H&E stained slide with the tumour region circled, and the estimated % tumour content written in the Tumour Content field of the requisition.  NOTE: A minimum of 10% tumour and at least 200 nuclei is required.
    • Specimen block and/or at least one unstained slide for each probe requested.

NOTE: FISH analysis on a bone marrow aspirate for the purposes of staging is not performed, unless a lymph node is not available


Results are reported within fourteen days from receipt of specimen and completed requisition form.  


  • Specimens are reported as Positive for a rearrangement, Negative for a rearrangement, or Negative for a rearrangement – extra signals


FISH analysis is performed on the provided specimen using the MYC (8q24) dual-colour, break-apart probe (Vysis). If the specimen is positive for a MYC rearrangement, then it is reflexed for further testing using the BCL2 (18q21) dual-colour, break-apart probe (Vysis) and the BCL6 (3q27) dual-colour, break-apart probe (Vysis).