OVERVIEW
High-grade B-cell lymphomas are tumors of B-cells with aggressive appearing histology that encompass a variety of disease entities using current WHO criteria, including diffuse large B-cell lymphoma, Burkitt lymphoma, high-grade B-cell lymphoma, NOS, etc. Importantly, it is necessary to identify and/or exclude the presence MYC and BCL2 and/or BCL6 rearrangements in these tumors as the results dictate therapy in most patients.
TEST REQUIREMENTS
- Completed CGL Lymphoid Testing requisition form
- FFPE Tumour specimen (see Specimen Guidelines, Cytogenetics FISH FFPE test type)
- An H&E stained slide with the tumour region circled, and the estimated % tumour content written in the Tumour Content field of the requisition. NOTE: A minimum of 10% tumour and at least 200 nuclei is required.
- Specimen block and/or at least one unstained slide for each probe requested.
- See our FFPE Guidelines for additional details.
NOTE: FISH analysis on a bone marrow aspirate for the purposes of staging is not performed, unless a lymph node is not available
TURN-AROUND TIME
Results are reported within fourteen days from receipt of specimen and completed requisition form.
RESULT REPORTING
- Specimens are reported as Positive for a rearrangement, Negative for a rearrangement, or Negative for a rearrangement – extra signals
METHOD
Sequential FISH analysis is performed using a MYC break-apart probe (Cytocell). If a MYC rearrangement is identified, reflex FISH analysis using a BCL2 and BCL6 FISH break-apart probes (Vysis). There must be a minimum of 200 tumour nuclei within each section or area to perform the analysis.
