OVERVIEW
Mantle cell lymphoma (MCL) is a B-cell neoplasm which comprises 3-10% of non-Hodgkin lymphoma. The common sites of involvement are lymph nodes, spleen, and bone marrow with or without peripheral blood involvement. The morphology of MCL is variable and a blastic variant does occur and mimics acute lymphoblastic leukemia.
Virtually all MCL have a t(11;14)(q13;q32) involving IGH and CCND1. Rare cases of CCND1 translocations with IGL chains (chromosomes 2 and 22) are recorded as well as cases without cyclin D1 expression that have a translocation involving CCND2 and CCND3 instead of CCND1.
TEST REQUIREMENTS
- Completed CGL Lymphoid Testing requisition form
- One of the following specimens (please see our Specimen Guidelines, Cytogenetics FISH test type:
- 4mL peripheral blood collected in sodium heparin tubes (green top)
- 2×1.0 mL of marrow aspirate in 9mL media (RPMI 1640, 3.8% FBS, antibiotics)
- Also accepted, but not preferred specimens types:
- Bone marrow biopsy in 9mL media (RPMI 1640, 3.8% FBS, antibiotics)
- NOTE: FFPE tissue has NOT been validated for this assay and is therefore not an acceptable specimen type
TURN-AROUND TIME
Results are reported within fourteen days from receipt of specimen and completed requisition form.
RESULTS REPORTING
- Specimens are reported as Positive for a rearrangement, Negative for a rearrangement
- The majority of MCL cases will have an IGH/CCND1 rearrangement identified by FISH, however, rare cases exist that have cryptic rearrangements or rearrangements involving CCND2 or CCND3
- IGH/CCNDI rearrangements can also be identified in multiple myeloma.
METHOD
The submitted BM or PB specimen is harvested into a methanol-acetic acid (MAA) fixed cell pellet. FISH analysis is performed using a dual colour, dual-fusion probe (Vysis) to IGH (14q32) and CCND1 (11q13).
REFERENCES
- Remstein et al. (2000) Br J Haematol . PMID: 11054068
- Belaud-Rotureau et al (2002) Mod Pathol PMID: 12011256
- Wlodarska et al (2008) Blood PMID: 18391076